Rbd-991 May 2026

: The synthesized fragment is ligated into the vector using kits like the ClonExpress® Ultra One Step Cloning Kit and then transformed into a host like E. coli XL1-Blue .

and subsequently purified (often using Ni Sepharose resin if His-tagged) to be used as a "piece" or immunogen in experiments. Contextual Alternatives RBD-991

In molecular biology, "RBD-991" identifies a DNA sequence fragment starting at the of the SARS-CoV-2 S protein gene (GenBank: NC_045512.2). : The synthesized fragment is ligated into the

: The target fragment (bases 991–1749) corresponding to amino acids 331–583 is synthesized to represent the Receptor-Binding Domain (RBD). Vector Digestion : An expression vector, such as pQE30 such as pQE30

, is prepared by digestion with restriction enzymes like and HindIII .